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Correspondence

Indian Pediatr 2018;55: 709-710

Galactomannan Antigen Test for Invasive Aspergillus Infection in Febrile Neutropenic Children: Authors' Reply

 

Rachna Seth* and Jogender Kumar

Department of Pediatrics, AIIMS, New Delhi, India.
Email: *[email protected]

    


We thank the authors for showing interest in our article. The clarifications to the concerns raised are as follows:

1. In the ‘proven’ category of invasive fungal infections, none of the isolate was Aspergillus. Fungi isolated from blood were Candida albicans, Candida tropicalis, and Trichosporon spp. 

2. Out of 11 isolates from sputum, A. fumigates was positive in seven and A. flavus was the species in four patients. The antimicrobial sensitivity details are not available.

3. The authors rightly stated that current literature argues against cross-reactivity of Galactomannan (GM) antigen test and the new EDTA-containing piperacillin/tazobactam formulation. However, in low- and middle-income countries where generic formulations are widely used, it remains a valid concern. A recent study had shown that the rate of false positive serum GM antigen test was as high as 56% in patients who received generic preparation [1]. With standard brands, the association is no longer applicable, but false positive can still be there with generic medicines [2].

4. We agree that pulmonary leukostasis may lead to a false impression of a fungal nodule or ground-glass opacities on computed tomograph (CT) scan. In our study, three patients had hyperleukocytosis but none of them had evidence of fungal infection on CT scan. 

5. We did not perform bronchoalveolar lavage (BAL) after recovery as baseline information on BAL was not available. We agree that GM antigen testing from BAL is more sensitive as well as specific for invasive fungal infections. 

References

1. Demiraslan H, Atalay MA, Eren E, Demir K, Kaynar L, Koc AN, et al. Assessing the risk of false positive serum galactomannan among patients receiving piperacillin/tazobactam for febrile neutropenia. Med Mycol. 2017;55:535-40. 

2. Gerlinger MP, Rousselot P, Rigaudeau S, Billon C, Touratier S, Castaigne S, et al. False positive galactomannan Platelia due to piperacillin-tazobactam. Médecine Mal Infect. 2012;42:10-4.

 

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